Nitrogen 400 calibration gas — 400 ppm Nitrogen Dioxide (NO₂) balance Nitrogen (N₂) — is one of the most searched calibration gas mixtures in the United States. This guide covers what it is, which detectors it calibrates, how to choose a cylinder size, and how to buy it direct with NIST-traceable documentation.
Buy Nitrogen 400 (NO₂ 400 ppm, 34L) — $220
NIST-traceable Certificate of Analysis included. Manufacturer-direct pricing — the same 34L disposable cylinder other brands resell for more.
The gas we supply is produced by laboratories accredited to ISO/IEC 17025:2017 and ISO 17034:2016. Every cylinder ships with a Certificate of Analysis traceable to NIST. Made in the USA — ships from our Florida facility in 48 hours.
What is Nitrogen 400 calibration gas?
“Nitrogen 400” is shorthand for a mixture of 400 ppm Nitrogen Dioxide (NO₂) balance Nitrogen (N₂), used as a reference standard to verify and calibrate instruments that measure toxic NO₂. It is commonly specified for industrial hygiene (welding fumes, engine exhaust, combustion byproducts), environmental testing, and fixed gas-detection systems.
Where is it used?
| Industry | Application | Typical detectors |
|---|---|---|
| Chemical manufacturing | NO₂ toxic gas monitoring | MSA, BW Technologies |
| Welding / fabrication | Fume exposure calibration | Honeywell, Draeger |
| Automotive / tunnels | Exhaust gas monitoring | RAE Systems, RKI |
| Power generation | Combustion analysis | Industrial Scientific |
| Mining | Blast fume clearance | Crowcon, GMI |
Available cylinder sizes
| Size | Part # | Connection | Best for |
|---|---|---|---|
| 34 L aluminum | 34L-111-400 | C-10 | Standard calibration — $220 |
| 58 L aluminum | 58L-111-400 | C-10 | High-frequency calibration |
| 105 L aluminum | 105L-111-400 | C-10 | Multiple instruments |
| 116 L aluminum | 116L-111-400 | C-10 | Lab / heavy use |
Balance-air versions (part numbers 34L/58L/105L/116L-112-400) are also available on request.
Cross-reference — replacing another brand
Trigas USA manufactures Nitrogen 400 to the same NIST-traceable specification as the 400 ppm NO₂ (“nitrogen 400”) disposable cylinders sold by Gasco distributors and Cal Gas Direct — same gas, same concentration, same disposable cylinder format, manufacturer-direct. Our part number family is 111-400 (e.g. 34L-111-400 for the 34 liter). If you have another supplier’s part number, use the part number finder or the OEM cross-reference pages and we’ll match your exact equivalent.
Trigas USA is independent and not affiliated with other brands; they are referenced only to help identify the equivalent product.
Why ISO 17025 accreditation matters
ISO/IEC 17025:2017 is the international standard for testing and calibration laboratories — it requires documented traceability to NIST, independent third-party audits, uncertainty quantification on every CoA, and verified technical competency. The gas we supply is produced in ISO 17025-accredited laboratories, so the documentation you receive meets OSHA, EPA Part 75, and pharmaceutical (FDA GMP) audit requirements.
Shelf life and storage
NO₂ is reactive and can degrade in storage. Shelf life: 12 months from the fill date (all sizes). Every cylinder shows manufacture and expiration dates. Store upright in a cool, dry place (4–40°C / 40–104°F), away from sunlight, heat, oxidizers, and ignition sources; secure against tipping.
How to order
- Single purchase: select Nitrogen 400 and your size at trigas-usa.com; CoA and SDS included.
- B2B / Net-30: purchase orders and volume pricing on 10+ cylinders — email sales@trigas-usa.com.
Shipping
Disposable calibration gas cylinders ship as DOT Division 2.2 (UN1956) hazardous materials via ground service with certified hazmat documentation, from our Florida facility. Most of the continental US receives orders in 1–5 business days.
FAQ
Is Nitrogen 400 the same as 400 ppm NO₂ in nitrogen? Yes.
What regulator do I need? A fixed-flow regulator with a C-10 connection. Because NO₂ is a reactive gas, a stainless steel regulator is recommended. Compatible regulators are available at trigas-usa.com.
How do I verify a cylinder is still good? Check the expiration date on the label; do not use expired gas. Email sales@trigas-usa.com for a replacement.
Questions? Email sales@trigas-usa.com.